Journal: Materials Today Bio
Article Title: Multiple-pathway cGAS-STING activation with enhanced mild photothermal therapy through glycolysis regulation for boosting gastric cancer immunotherapy
doi: 10.1016/j.mtbio.2026.102790
Figure Lengend Snippet: In vitro antitumor efficacy of the nanoparticles, ICD induction, and activation of the cGAS-STING pathway. Uptake of MOMn and MOMn@MB by MFC cells at (a) 4 and (b) 6 h. (c) Calcein-AM/PI and (d) JC-1 staining fluorescence images of MFC cells after treatment with different groups. Cytotoxicity analysis of MFC cells treated with (e) MO@MB and (f) MOMn@MB. Western blot analysis of (g) glycolysis pathway related proteins and (h) HSP70/90 in MFC cells treated with different nanoparticles. (i) CLSM images of CRT exposure on the surface of MFC cells after different treatments. (j) DNA damage assessment via γ-H2AX immunostaining in MFC cells treated with different nanoparticles. Levels of (k) lactate, (l) ATP, (m) HMGB1, and (n) IFN-β in MFC cells treated with different nanoparticles. (o) Western blot analysis of cGAS-STING pathway related proteins with different treatment. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. G1-G6 are denoted as Control, NIR Laser, MO@MB, MO@MB + NIR Laser, MOMn@MB, and MOMn@MB + NIR Laser groups, respectively.
Article Snippet: The mouse HMGB1 enzyme-linked immunosorbent assay (ELISA) kit and mouse interferon-β (IFN-β) ELISA kit were purchased from Wuhan Elabscience Biotechnology Co., Ltd.
Techniques: In Vitro, Activation Assay, Staining, Fluorescence, Western Blot, Immunostaining, Control