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mouse interferon beta ifn β enzyme linked immunosorbent assay  (Elabscience Biotechnology)


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    Elabscience Biotechnology mouse interferon beta ifn β enzyme linked immunosorbent assay
    Mouse Interferon Beta Ifn β Enzyme Linked Immunosorbent Assay, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse interferon beta ifn β enzyme linked immunosorbent assay/product/Elabscience Biotechnology
    Average 95 stars, based on 45 article reviews
    mouse interferon beta ifn β enzyme linked immunosorbent assay - by Bioz Stars, 2026-04
    95/100 stars

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    In vitro antitumor efficacy of the nanoparticles, ICD induction, and activation of the cGAS-STING pathway. Uptake of MOMn and MOMn@MB by MFC cells at (a) 4 and (b) 6 h. (c) Calcein-AM/PI and (d) JC-1 staining fluorescence images of MFC cells after treatment with different groups. Cytotoxicity analysis of MFC cells treated with (e) MO@MB and (f) MOMn@MB. Western blot analysis of (g) glycolysis pathway related proteins and (h) HSP70/90 in MFC cells treated with different nanoparticles. (i) CLSM images of CRT exposure on the surface of MFC cells after different treatments. (j) DNA damage assessment via γ-H2AX immunostaining in MFC cells treated with different nanoparticles. Levels of (k) lactate, (l) ATP, (m) HMGB1, and <t>(n)</t> <t>IFN-β</t> in MFC cells treated with different nanoparticles. (o) Western blot analysis of cGAS-STING pathway related proteins with different treatment. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. G1-G6 are denoted as Control, NIR Laser, MO@MB, MO@MB + NIR Laser, MOMn@MB, and MOMn@MB + NIR Laser groups, respectively.
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    In vitro antitumor efficacy of the nanoparticles, ICD induction, and activation of the cGAS-STING pathway. Uptake of MOMn and MOMn@MB by MFC cells at (a) 4 and (b) 6 h. (c) Calcein-AM/PI and (d) JC-1 staining fluorescence images of MFC cells after treatment with different groups. Cytotoxicity analysis of MFC cells treated with (e) MO@MB and (f) MOMn@MB. Western blot analysis of (g) glycolysis pathway related proteins and (h) HSP70/90 in MFC cells treated with different nanoparticles. (i) CLSM images of CRT exposure on the surface of MFC cells after different treatments. (j) DNA damage assessment via γ-H2AX immunostaining in MFC cells treated with different nanoparticles. Levels of (k) lactate, (l) ATP, (m) HMGB1, and <t>(n)</t> <t>IFN-β</t> in MFC cells treated with different nanoparticles. (o) Western blot analysis of cGAS-STING pathway related proteins with different treatment. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. G1-G6 are denoted as Control, NIR Laser, MO@MB, MO@MB + NIR Laser, MOMn@MB, and MOMn@MB + NIR Laser groups, respectively.
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    In vitro antitumor efficacy of the nanoparticles, ICD induction, and activation of the cGAS-STING pathway. Uptake of MOMn and MOMn@MB by MFC cells at (a) 4 and (b) 6 h. (c) Calcein-AM/PI and (d) JC-1 staining fluorescence images of MFC cells after treatment with different groups. Cytotoxicity analysis of MFC cells treated with (e) MO@MB and (f) MOMn@MB. Western blot analysis of (g) glycolysis pathway related proteins and (h) HSP70/90 in MFC cells treated with different nanoparticles. (i) CLSM images of CRT exposure on the surface of MFC cells after different treatments. (j) DNA damage assessment via γ-H2AX immunostaining in MFC cells treated with different nanoparticles. Levels of (k) lactate, (l) ATP, (m) HMGB1, and <t>(n)</t> <t>IFN-β</t> in MFC cells treated with different nanoparticles. (o) Western blot analysis of cGAS-STING pathway related proteins with different treatment. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. G1-G6 are denoted as Control, NIR Laser, MO@MB, MO@MB + NIR Laser, MOMn@MB, and MOMn@MB + NIR Laser groups, respectively.
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    Image Search Results


    In vitro antitumor efficacy of the nanoparticles, ICD induction, and activation of the cGAS-STING pathway. Uptake of MOMn and MOMn@MB by MFC cells at (a) 4 and (b) 6 h. (c) Calcein-AM/PI and (d) JC-1 staining fluorescence images of MFC cells after treatment with different groups. Cytotoxicity analysis of MFC cells treated with (e) MO@MB and (f) MOMn@MB. Western blot analysis of (g) glycolysis pathway related proteins and (h) HSP70/90 in MFC cells treated with different nanoparticles. (i) CLSM images of CRT exposure on the surface of MFC cells after different treatments. (j) DNA damage assessment via γ-H2AX immunostaining in MFC cells treated with different nanoparticles. Levels of (k) lactate, (l) ATP, (m) HMGB1, and (n) IFN-β in MFC cells treated with different nanoparticles. (o) Western blot analysis of cGAS-STING pathway related proteins with different treatment. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. G1-G6 are denoted as Control, NIR Laser, MO@MB, MO@MB + NIR Laser, MOMn@MB, and MOMn@MB + NIR Laser groups, respectively.

    Journal: Materials Today Bio

    Article Title: Multiple-pathway cGAS-STING activation with enhanced mild photothermal therapy through glycolysis regulation for boosting gastric cancer immunotherapy

    doi: 10.1016/j.mtbio.2026.102790

    Figure Lengend Snippet: In vitro antitumor efficacy of the nanoparticles, ICD induction, and activation of the cGAS-STING pathway. Uptake of MOMn and MOMn@MB by MFC cells at (a) 4 and (b) 6 h. (c) Calcein-AM/PI and (d) JC-1 staining fluorescence images of MFC cells after treatment with different groups. Cytotoxicity analysis of MFC cells treated with (e) MO@MB and (f) MOMn@MB. Western blot analysis of (g) glycolysis pathway related proteins and (h) HSP70/90 in MFC cells treated with different nanoparticles. (i) CLSM images of CRT exposure on the surface of MFC cells after different treatments. (j) DNA damage assessment via γ-H2AX immunostaining in MFC cells treated with different nanoparticles. Levels of (k) lactate, (l) ATP, (m) HMGB1, and (n) IFN-β in MFC cells treated with different nanoparticles. (o) Western blot analysis of cGAS-STING pathway related proteins with different treatment. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. G1-G6 are denoted as Control, NIR Laser, MO@MB, MO@MB + NIR Laser, MOMn@MB, and MOMn@MB + NIR Laser groups, respectively.

    Article Snippet: The mouse HMGB1 enzyme-linked immunosorbent assay (ELISA) kit and mouse interferon-β (IFN-β) ELISA kit were purchased from Wuhan Elabscience Biotechnology Co., Ltd.

    Techniques: In Vitro, Activation Assay, Staining, Fluorescence, Western Blot, Immunostaining, Control

    DC maturation and activation of the cGAS-STING pathway. (a) Schematic diagram of the extraction from BMDCs to DC maturation and the Transwell assay. Created by Biorender. (b) Expression of CD80 and CD86 quantitatively determined by flow cytometry analysis with different treatments, and (c) the average DC maturation rate based on the results. (d) The levels of IFN-β in DC cells with different treatments. (e) Western blot analysis was employed to evaluate the expression of cGAS-STING pathway-related proteins in DC cells across different treatments. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. G1-G6 are denoted as Control, NIR Laser, MO@MB, MO@MB + NIR Laser, MOMn@MB, and MOMn@MB + NIR Laser groups, respectively.

    Journal: Materials Today Bio

    Article Title: Multiple-pathway cGAS-STING activation with enhanced mild photothermal therapy through glycolysis regulation for boosting gastric cancer immunotherapy

    doi: 10.1016/j.mtbio.2026.102790

    Figure Lengend Snippet: DC maturation and activation of the cGAS-STING pathway. (a) Schematic diagram of the extraction from BMDCs to DC maturation and the Transwell assay. Created by Biorender. (b) Expression of CD80 and CD86 quantitatively determined by flow cytometry analysis with different treatments, and (c) the average DC maturation rate based on the results. (d) The levels of IFN-β in DC cells with different treatments. (e) Western blot analysis was employed to evaluate the expression of cGAS-STING pathway-related proteins in DC cells across different treatments. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. G1-G6 are denoted as Control, NIR Laser, MO@MB, MO@MB + NIR Laser, MOMn@MB, and MOMn@MB + NIR Laser groups, respectively.

    Article Snippet: The mouse HMGB1 enzyme-linked immunosorbent assay (ELISA) kit and mouse interferon-β (IFN-β) ELISA kit were purchased from Wuhan Elabscience Biotechnology Co., Ltd.

    Techniques: Activation Assay, Extraction, Transwell Assay, Expressing, Flow Cytometry, Western Blot, Control